Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human thyroid cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66034-1-Ig targets TTF1/NKX2-1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | NK2 homeobox 1 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | NM_003317 |
| Gene Symbol | TTF1 |
| Gene ID (NCBI) | 7080 |
| RRID | AB_11045663 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43699 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TTF1/NKX2-1 antibody 66034-1-Ig | Download protocol |
| IHC protocol for TTF1/NKX2-1 antibody 66034-1-Ig | Download protocol |
| WB protocol for TTF1/NKX2-1 antibody 66034-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TTF1/NKX2-1 Monoclonal Antibody (2C8F3), catalog number 66034-1-Ig, has accumulated 15 citations published in journals including Nature Communications, Advanced Science, Cancer Biology & Medicine, Free Radical Biology and Medicine, British Journal of Cancer, Frontiers in Immunology, International Journal of Molecular Sciences, ACS Omega, and Molecular Pharmaceutics. The associated research fields encompass oncology (lung, gastric, and thyroid cancers), immunology, cell biology (organoid modeling, neural stem cells), endocrinology, and pharmacology.
| Species | Application | Title |
|---|---|---|
Nat Commun Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features | ||
Nat Commun Intrinsic resistance to RAS inhibitors is driven by dysregulation of KRAS degradation. | ||
Adv Sci (Weinh) Excessive MYC Orchestrates Macrophages induced Chromatin Remodeling to Sustain Micropapillary-Patterned Malignancy in Lung Adenocarcinoma | ||
Cancer Biol Med SARS-CoV-2 infection in brain tumors and the association with alterations in the tumor immune microenvironment. | ||
Free Radic Biol Med GnIH-induced mitochondrial dysfunction lead to oxidative stress and apoptosis in thyroid follicular cells, causing hypothyroidism. | ||
Br J Cancer Novel transcription factor zinc finger 514 suppresses lung adenocarcinoma progression and enhances cisplatin sensitivity via transcriptional repression of COL1A1. |
