Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66151-1-Ig targets HBE1-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | hemoglobin, epsilon 1 |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | NM_005330 |
| Gene Symbol | HBE1 |
| Gene ID (NCBI) | 3046 |
| RRID | AB_2881547 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02100 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HBE1-Specific antibody 66151-1-Ig | Download protocol |
| WB protocol for HBE1-Specific antibody 66151-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HBE1-Specific Monoclonal antibody (2C11G6), catalog number 66151-1-Ig, has a total of 1 citation. The citation appears in Cell Reports (2025), in a study titled "A post-implantation model of human embryo development includes a definitive hematopoietic niche." The research field associated with this citation is human embryonic development and hematopoiesis, specifically examining the role of epsilon hemoglobin (HBE1) as a marker for fetal nucleated red blood cells within a post-implantation embryo model. The antibody was applied via immunofluorescence (IF) on human samples.
| Species | Application | Title |
|---|---|---|
Cell Rep A post-implantation model of human embryo development includes a definitive hematopoietic niche. |
