Tested Applications
| Positive WB detected in | human kidney tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66174-1-Ig targets TPSAB1/TPSB2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4167 Product name: Recombinant human TPSAB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC028059 Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP Predict reactive species |
| Full Name | tryptase alpha/beta 1 |
| Calculated Molecular Weight | 275 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC028059 |
| Gene Symbol | TPSAB1 |
| Gene ID (NCBI) | 7177 |
| RRID | AB_2881569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15661 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TPSAB1(Tryptase alpha/beta-1) is also named as TPS1, TPS2, TPSB1 and belongs to the peptidase S1 family. It is the major neutral protease present in mast cells and secreted upon the coupled activation-degranulation response of this cell type. TPSAB1 has 2 isoforms produced by alternative splicing with the molecular weight of 31 kDa and 29 kDa. This full length protein has two glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TPSAB1/TPSB2 antibody 66174-1-Ig | Download protocol |
| WB protocol for TPSAB1/TPSB2 antibody 66174-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TPSAB1/TPSB2 Monoclonal antibody (6A10G1), catalog number 66174-1-Ig, has 3 citations. These appear in Molecular Psychiatry, Oncogene, and International Immunopharmacology. The research fields span autism spectrum disorder (hippocampal molecular profiling), colorectal cancer (mast cell–mediated ER stress), and rosacea (mast cell degranulation and autophagy). Applications cited include IHC and WB in human samples.
| Species | Application | Title |
|---|---|---|
Mol Psychiatry Molecular profiling of the hippocampus of children with autism spectrum disorder | ||
Oncogene Mast cells inhibit colorectal cancer development by inducing ER stress through secreting Cystatin C | ||
Int Immunopharmacol Activation of aryl hydrocarbon receptor ameliorates degranulation of LL-37 induced mast cells in rosacea through enhancing autophagy |
Reviews
What customers say
One reviewer used this antibody for immunohistochemistry on human FFPE tonsillitis tissue, employing heat-induced epitope retrieval with citrate buffer at a 1:200 dilution. The product received a 4 out of 5 rating, indicating generally positive performance for IHC applications on formalin-fixed, paraffin-embedded human tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Santhosh (Verified Customer) (04-15-2019) | Human FFPE tonsilitis tissue. HIER with citrate buffer
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