Tested Applications
| Positive WB detected in | fetal human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66228-1-Ig targets RND3 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18839 Product name: Recombinant human RND3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-244 aa of BC012513 Sequence: MKERRASQKLSSKSIMDPNQNVKCKIVVVGDSQCGKTALLHVFAKDCFPENYVPTVFENYTASFEIDTQRIELSLWDTSGSPYYDNVRPLSYPDSDAVLICFDISRPETLDSVLKKWKGEIQEFCPNTKMLLVGCKSDLRTDVSTLVELSNHRQTPVSYDQGANMAKQIGAATYIECSALQSENSVRDIFHVATLACVNKTNKNVKRNKSQRATKRISHMPSRPELSAVATDLRKDKAKSCTVM Predict reactive species |
| Full Name | Rho family GTPase 3 |
| Calculated Molecular Weight | 244 aa, 27 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC012513 |
| Gene Symbol | RND3 |
| Gene ID (NCBI) | 390 |
| RRID | AB_2881619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61587 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rnd3, also known as RhoE, belongs to the Rnd subclass of the Rho family of small guanosine triphosphate (GTP)-binding proteins. Rnd proteins are unique due to their inability to switch from a GTP-bound to GDP-bound conformation. The basic role of Rnd3 is to report as an endogenous antagonist of RhoA signaling-mediated actin cytoskeleton dynamics, which specifically contributes to cell migration and neuron polarity. Rnd3 also plays a critical role in arresting cell cycle distribution, inhibiting cell growth, and inducing apoptosis and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RND3 antibody 66228-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RND3 Monoclonal antibody (3H8F9), catalog number 66228-1-Ig, has 7 total citations. These appear in journals including Cell Communications and Signaling, International Journal of Molecular Medicine, Molecular Therapy Nucleic Acids, Journal of Biological Chemistry, Microbiology Spectrum, Virology, and bioRxiv. Research fields span prostate cancer cytoskeletal regulation, cardiomyocyte signaling pathways, preeclampsia/trophoblast biology, hepatocellular carcinoma cholesterol metabolism, viral replication and interferon responses, and coronavirus infection transcriptomics.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal The hexosamine biosynthetic pathway alters the cytoskeleton to modulate cell proliferation and migration in aggressive prostate cancer. | ||
Int J Mol Med Ingenuity pathway analysis of differentially expressed genes involved in signaling pathways and molecular networks in RhoE gene‑edited cardiomyocytes.
| ||
Mol Ther Nucleic Acids Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE. | ||
J Biol Chem RhoE downregulation leads to enhanced cholesterol biosynthesis and sorafenib resistance in hepatocellular carcinoma.
| ||
Microbiol Spectr RND3 restricts encephalomyocarditis virus replication by promoting IKKε ubiquitination and type I interferon production.
| ||
Virology Transcriptomic analysis reveals crucial regulatory roles of immediate-early response genes and related signaling pathways in coronavirus infectious bronchitis virus infection |
Reviews
What customers say
One customer rated this antibody 4 out of 5 stars for Western Blot at a 1:1000 dilution. They used it on U937 and PBMC cells and reported that it is of very good quality and successfully detects RND3 in both cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yuan (Verified Customer) (12-10-2020) | This antibody is very good quality and can detect RND3 in both U937 and PBMC cells.
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