Tested Applications
| Positive WB detected in | Neuro-2a cells, HEK-293 cells, PC-12 cells, rat cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66259-1-Ig targets Nestin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nestin |
| Calculated Molecular Weight | 177 kDa |
| Observed Molecular Weight | 150-170 kDa |
| GenBank Accession Number | NM_006617 |
| Gene Symbol | Nestin |
| Gene ID (NCBI) | 10763 |
| RRID | AB_2756523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P48681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nestin antibody 66259-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Nestin Monoclonal antibody (catalog number 66259-1-Ig) has 44 citations. These appear in journals including Cell Metabolism, Cell Research, Cell Death & Disease, Small, Journal of Allergy and Clinical Immunology, EBioMedicine, Acta Biomaterialia, Cell Reports, CNS Neuroscience & Therapeutics, Cell Biosciences, Life Sciences, Cells, Molecular Neurobiology, Scientific Reports, Cancers, Frontiers in Cell and Developmental Biology, and Journal of Cancer. Research fields span neural stem cells, neurogenesis, glioma/glioblastoma, osteoarthritis, spinal cord injury, and stem cell biology.
| Species | Application | Title |
|---|---|---|
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
Cell Res RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability. | ||
Cell Death Dis Nonenzymatic function of Aldolase A downregulates miR-145 to promote the Oct4/DUSP4/TRAF4 axis and the acquisition of lung cancer stemness. | ||
Small Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect. | ||
J Allergy Clin Immunol Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1. | ||
EBioMedicine Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke. |
