Tested Applications
| Positive WB detected in | human spleen tissue, pig spleen tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66262-1-Ig targets AIRE in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19103 Product name: Recombinant human AIRE protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 96-348 aa of BC137270 Sequence: QKNEDECAVCRDGGELICCDGCPRAFHLACLSPPLREIPSGTWRCSSCLQATVQEVQPRAEEPRPQEPPVETPLPPGLRSAGEEVRGPPGEPLAGMDTTLVYKHLPAPPSAAPLPGLDSSALHPLLCVGPEGQQNLAPGARCGVCGDGTDVLRCTHCAAAFHWRCHFPAGTSRPGTGLRCRSCSGDVTPAPVEGVLAPSPARLAPGPAKDDTASHEPALHRDDLESLLSEHTFDGILQWAIQSMARPAAPFPS Predict reactive species |
| Full Name | autoimmune regulator |
| Calculated Molecular Weight | 545 aa, 58 kDa |
| Observed Molecular Weight | 58-69 kDa |
| GenBank Accession Number | BC137270 |
| Gene Symbol | AIRE |
| Gene ID (NCBI) | 326 |
| RRID | AB_2881649 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43918 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AIRE antibody 66262-1-Ig | Download protocol |
| WB protocol for AIRE antibody 66262-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this antibody reports excellent performance in immunohistochemistry and immunofluorescence on human frozen thymus tissue, with the reviewer describing it as working "beautifully" and awarding a full 5-star rating.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fla (Verified Customer) (02-05-2026) | The antybody works beautifully on human frozen thymus
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