Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, ROS1728 cells, HSC-T6 cells. RAW 264.7 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | HeLa cells, MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66289-1-Ig targets Pan Acetylation in WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA, Acetylation assay applications and shows reactivity with chemical compound samples.
| Tested Reactivity | chemical compound |
| Cited Reactivity | human, mouse, rat, drosophila, rice |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Pan Acetylation |
| Observed Molecular Weight | 17 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881672 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acetylation and De-acetylation are the important reaction in epigenetic. Many proteins, especially Histone, are with Acetylated of Lysine. This antibody is used to detect the Acetylation of Lysine. The 17 kDa in our detection is Acetylated Histone.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Pan Acetylation antibody 66289-1-Ig | Download protocol |
| WB protocol for Pan Acetylation antibody 66289-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Pan Acetylation Monoclonal antibody (1D5F2), Cat# 66289-1-Ig, has 94 citations published in journals including Nature Communications, Neuron, Molecular Cell, EMBO Journal, Cell Reports, FASEB Journal, Cancer Research, and others. Associated research fields span epigenetics (histone acetylation/lactylation), oncology (breast, colorectal, liver, lung, prostate cancers), metabolism (lipid, NAD⁺, glucose), neurodegeneration (Alzheimer's, Parkinson's), cardiovascular disease, immunology, ferroptosis, autophagy, and aging.
| Species | Application | Title |
|---|---|---|
Adv Mater Disrupting KAT8 Liquid-Liquid Phase Separation with Hybrid Vesicle-Liposome Platform for Enhanced PD-L1 Blockade Treatment. | ||
Cancer Res PRODH2-Mediated Metabolism in the Bone Microenvironment Promotes Breast Cancer Metastasis. | ||
Nat Commun Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes. | ||
Nat Commun Exercise-induced anti-obesity effects in male mice generated by a FOXO1-KLF10 reinforcing loop promoting adipose lipolysis | ||
Nat Commun Multi-protomics analysis identified host cellular pathways perturbed by tick-borne encephalitis virus infection | ||
Nat Commun MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot at a 1:1000 dilution. One user tested it on liver tissue and reported it works well for Western blot. The other tested it on 293T cells and noted it gives a nice, strong, and specific signal. Overall, the antibody is praised for its strong, specific performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (10-24-2025) | works well for Western blot
|
Xiaoyu (Verified Customer) (07-18-2024) | Gives a nice, strong and specific signal in Western Blot.
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