Tested Applications
| Positive WB detected in | human skeletal muscle tissue |
| Positive FC (Intra) detected in | NK92, NK92 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66368-1-Ig targets NFIL3 in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2380 Product name: Recombinant human NFIL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 113-462 aa of BC008197 Sequence: ATLKAELLSLKLKFGLISSTAYAQEIQKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHSPQSSLSDVSEVSSVEHTQESSVQGSCRSPENKFQIIKQEPMELESYTREPRDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDGVVGKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEFEATQKLSSPIDMTSKRHFELEKHSAPSMVHSSLTPFSVQVTNIQDWSLKSEHWHQKELSGKTQNSFKTGVVEMKDSGYKVSDPENLYLKQGIANLSAEVVSLKRLIATQPISASDSG Predict reactive species |
| Full Name | nuclear factor, interleukin 3 regulated |
| Calculated Molecular Weight | 462 aa, 51 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC008197 |
| Gene Symbol | NFIL3 |
| Gene ID (NCBI) | 4783 |
| RRID | AB_2881748 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16649 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NFIL3 antibody 66368-1-Ig | Download protocol |
| WB protocol for NFIL3 antibody 66368-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NFIL3 Monoclonal antibody (2F4B4), catalog number 66368-1-Ig, has a total of 1 citation. The citation appears in Cell Death & Disease (Impact Factor: 12.2, published February 2025). The associated research field involves acute kidney injury (AKI), specifically examining the role of tubular CD44 in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction. The antibody was used for immunofluorescence (IF) on human samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Tubular CD44 plays a key role in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction |
