Tested Applications
| Positive WB detected in | HEK-293 cells, ROS1728 cells |
| Positive IHC detected in | human heart tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66388-1-Ig targets VDAC2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | voltage-dependent anion channel 2 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | NM_003375 |
| Gene Symbol | VDAC2 |
| Gene ID (NCBI) | 7417 |
| RRID | AB_2881764 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P45880 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VDAC2 antibody 66388-1-Ig | Download protocol |
| IHC protocol for VDAC2 antibody 66388-1-Ig | Download protocol |
| WB protocol for VDAC2 antibody 66388-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VDAC2 Monoclonal antibody (1D8C11), catalog number 66388-1-Ig, has 7 total citations. These appear in journals including Cancer Discovery, Molecular Cell, Phytomedicine, Cell Reports, Journal of Ethnopharmacology, Drug Development Research, and Cell Division. The cited research spans fields such as ferroptosis in BRCA1-deficient cancers, Parkin-mediated mitophagy, mitochondrial dysfunction in endotoxemia, lung adenocarcinoma, IgA nephropathy, non-small cell lung cancer, and cutaneous squamous cell carcinoma.
| Species | Application | Title |
|---|---|---|
Cancer Discov BRCA1-mediated dual regulation of ferroptosis exposes a vulnerability to GPX4 and PARP co-inhibition in BRCA1-deficient cancers | ||
Phytomedicine Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma. | ||
Cell Rep Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia | ||
J Ethnopharmacol Exploring the therapeutic effects and molecular mechanisms of total flavonoids of Abelmoschus manihot (L.) Medic in the treatment of IgA nephropathy based on WGCNA | ||
Drug Dev Res Xanthatin inhibits non-small cell lung cancer proliferation by breaking the redox balance. |
