Tested Applications
| Positive WB detected in | HeLa cells, SW 1990 cells, HGC-27 cells, MKN-45 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66404-1-Ig targets Mesothelin in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23606 Product name: Recombinant human MSLN protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 273-621 aa of BC003512 Sequence: RSSRDPSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSVQEALSGTPCLLGPGPVLTVLALLLASTLA Predict reactive species |
| Full Name | mesothelin |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 69 kDa, 40 kDa |
| GenBank Accession Number | BC003512 |
| Gene Symbol | MSLN |
| Gene ID (NCBI) | 10232 |
| RRID | AB_2881778 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13421 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Mesothelin antibody 66404-1-Ig | Download protocol |
| WB protocol for Mesothelin antibody 66404-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Mesothelin Monoclonal antibody (2E3A4), catalog number 66404-1-Ig, has a total of 3 citations. These appear in Journal of Experimental & Clinical Cancer Research, Journal of Translational Medicine, and Frontiers in Oncology. The associated research fields include brain metastasis of non-small cell lung cancer via MET activation, epithelial-mesenchymal transition through WNT5A signaling in colorectal cancer, and MSLN-positive circulating tumor cells as a diagnostic biomarker in epithelial ovarian cancer.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Mesothelin promotes brain metastasis of non-small cell lung cancer by activating MET | ||
J Transl Med Single-cell and spatial transcriptome profiling reveal CTHRC1+ fibroblasts promote EMT through WNT5A signaling in colorectal cancer | ||
Front Oncol Insights into the role of MSLN-positive circulating tumor cell as an auxiliary diagnostic biomarker in epithelial ovarian cancer |
Reviews
What customers say
The single review for 66404-1-Ig is from Boyan Zhang (October 2021), who rated it 5 stars. They used the antibody for Western Blot (lot 10004513) and reported that it recognized a strong band around 65 kDa, consistent with what is shown on the website. Overall, the reviewer was satisfied with the product's performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (10-05-2021) | This antibody recognized a strong band around 65 kd, which is as shown on the website
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