Tested Applications
| Positive WB detected in | A375 cells, human testis tissue, NCCIT cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66426-1-Ig targets SPARC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7390 Product name: Recombinant human SPARC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC004974 Sequence: MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI Predict reactive species |
| Full Name | secreted protein, acidic, cysteine-rich (osteonectin) |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 38 kDa, 52 kDa |
| GenBank Accession Number | BC004974 |
| Gene Symbol | SPARC |
| Gene ID (NCBI) | 6678 |
| RRID | AB_2881797 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09486 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SPARC antibody 66426-1-Ig | Download protocol |
| IHC protocol for SPARC antibody 66426-1-Ig | Download protocol |
| WB protocol for SPARC antibody 66426-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SPARC Monoclonal antibody (catalog no. 66426-1-Ig) has 10 citations. These appear in journals including Advanced Science, Acta Biomaterialia, Acta Pharmacologica Sinica, British Journal of Cancer, Frontiers in Endocrinology, Scientific Reports, International Journal of Cancer, Pathology Research and Practice, Experimental Cell Research, and Thoracic Cancer. Research fields span Crohn's disease, endometriosis, myocardial infarction, nasopharyngeal carcinoma, diabetic retinopathy, gastric cancer, pancreatic adenocarcinoma, renal cell carcinoma, tumor angiogenesis, and mesothelioma, with applications mainly in WB and IHC.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway | ||
Acta Pharmacol Sin Madecassoside mitigates acute myocardial infarction injury by activating the PKCB/SPARC signaling pathway
| ||
Acta Biomater Targeting Delivery of Mifepristone to Endometrial Dysfunctional Macrophages for Endometriosis Therapy | ||
Br J Cancer Inactivation of 3-hydroxybutyrate dehydrogenase type 2 promotes proliferation and metastasis of nasopharyngeal carcinoma by iron retention. | ||
Front Endocrinol (Lausanne) Secreted Protein Acidic and Rich in Cysteine Mediates the Development and Progression of Diabetic Retinopathy. | ||
Sci Rep miR-29a-3p regulates SPARC to inhibit ferroptosis and promote cell proliferation in gastric cancer. |
