Filter: AllWBIHCIF-P

WB analysis of human cerebellum using 66439-1-Ig
human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 66439-1-Ig
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 66439-1-Ig
human spleen tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 66439-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 66439-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 66439-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 66439-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens).
IF Staining of mouse brain using 66439-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of mouse brain using 66439-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

KChIP1 Monoclonal antibody

Name: KChIP1 Monoclonal antibody
Brand: Proteintech
SKU: 66439-1-Ig

KChIP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66439-1-Ig

Clone No.3D6C1

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human cerebellum tissue, fetal human brain tissue, human spleen tissue
Positive IHC detected in	human brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66439-1-Ig targets KChIP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5494 Product name: Recombinant human KCNIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-216 aa of BC050375 Sequence: MGAVMGTFSSLQTKQRRPSKDKIEDELEMTMVCHRPEGLEQLEAQTNFTKRELQVLYRGFKNECPSGVVNEDTFKQIYAQFFPHGDASTYAHYLFNAFDTTQTGSVKFEDFVTALSILLRGTVHEKLRWTFNLYDINKDGYINKEEMMDIVKAIYDMMGKYTYPVLKEDTPRQHVDVFFQKMDKNKDGIVTLDEFLESCQEDDNIMRSLQLFQNVM Predict reactive species
Full Name	Kv channel interacting protein 1
Calculated Molecular Weight	227 aa, 27 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC050375
Gene Symbol	KCNIP1
Gene ID (NCBI)	30820
RRID	AB_2881809
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9NZI2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

Protocols

Product Specific Protocols
IF protocol for KChIP1 antibody 66439-1-Ig	Download protocol
IHC protocol for KChIP1 antibody 66439-1-Ig	Download protocol
WB protocol for KChIP1 antibody 66439-1-Ig	Download protocol