Tested Applications
| Positive WB detected in | LNCaP cells, RAW 264.7 cells, HSC-T6 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, Neuro-2a cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive IF-Fro detected in | mouse breast cancer |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66607-1-Ig targets KU70/XRCC6 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21301 Product name: Recombinant human KU70,XRCC6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 307-609 aa of BC008343 Sequence: TGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD Predict reactive species |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC008343 |
| Gene Symbol | KU70 |
| Gene ID (NCBI) | 2547 |
| RRID | AB_2881967 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P12956 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for KU70/XRCC6 antibody 66607-1-Ig | Download protocol |
| IF protocol for KU70/XRCC6 antibody 66607-1-Ig | Download protocol |
| IHC protocol for KU70/XRCC6 antibody 66607-1-Ig | Download protocol |
| WB protocol for KU70/XRCC6 antibody 66607-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Ku70 antibody (Cat# 66607-1-Ig) has 16 citations published in journals including Immunity, Neuron, Autophagy, Nucleic Acids Research, Cell Reports, Oncogene, Cancer Letters, and iScience. Research fields span DNA double-strand break repair, cGAS-STING immune signaling, cancer radioresistance, aging-related inflammation, autophagy, blood-brain barrier integrity, chromatin biology, and myositis. Applications include WB, IF, IP, CoIP, ChIP, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Immunity Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation. | ||
Autophagy ATG9 inhibits rickettsia binding to the Host cell surface by blocking the rOmpB-XRCC6/KU70 interaction | ||
J Nanobiotechnology Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles. | ||
Nucleic Acids Res Interactome of intact chromatosome variants with site-specifically ubiquitylated and acetylated linker histone H1.2 | ||
Acta Pharm Sin B Cytoplasmic relocation of nuclear Ku70 by Bruceine A augments cGAS-STING pathway-mediated anti-tumor immunity. |
Reviews
What customers say
All three reviews are 5-star. Users report clear, specific bands in Western Blot across multiple cell types (lymphoblastoid, U2OS, 293T). One reviewer noted the antibody works effectively with both plasmid-expressed and endogenous protein, and also performed well in Immunofluorescence, Immunoprecipitation, and Cell culture applications. Overall, reviewers describe the antibody as reliable and producing strong, clean results at dilutions ranging from 1:10,000 to 1:50,000.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ba^tiste (Verified Customer) (08-28-2026) | Clear band, work well
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Alexandra (Verified Customer) (07-02-2024) | It works pretty effectively both with protein, expressed from plasmid and endogeous protein as well.
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Katherine (Verified Customer) (09-25-2023) | Band was clear!
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