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What's the best dilution for MT-ND5 in ?
Tested Applications
| Positive WB detected in | A431 cells, L02 cells, HepG2 cells, COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66613-1-Ig targets MT-ND5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | NADH dehydrogenase, subunit 5 (complex I) |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | AF465942 |
| Gene Symbol | MT-ND5 |
| Gene ID (NCBI) | 4540 |
| RRID | AB_2881973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P03915 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MT-ND5 antibody 66613-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MT-ND5 Monoclonal antibody (1C12G8), catalog number 66613-1-Ig, has a total of 2 citations. These appear in EMBO Reports and Molecular Medicine Reports. The cited research spans oncology and mitochondrial biology, specifically examining NRF1 activation in colorectal cancer progression and the anti-tumor effects of a PD-L1 inhibitor on mitochondrial function in melanoma cells. Both studies utilized the antibody for Western blot analysis in human samples.
| Species | Application | Title |
|---|---|---|
EMBO Rep LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1. | ||
Mol Med Rep Direct effects of the small molecule PD‑L1 inhibitor BMS‑202 on A375 melanoma cells: Anti‑tumor activity accompanied by increased mitochondrial function |
