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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HSC-T6 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| RIP | See 1 publications below |
Product Information
66615-1-Ig targets Pyruvate Carboxylase in WB, IHC, RIP, ELISA applications and shows reactivity with Human, Rat , mouse samples.
| Tested Reactivity | Human, Rat , mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8846 Product name: Recombinant human PC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 828-1178 aa of BC011617 Sequence: MERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE Predict reactive species |
| Full Name | pyruvate carboxylase |
| Calculated Molecular Weight | 1178 aa, 130 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC011617 |
| Gene Symbol | Pyruvate Carboxylase |
| Gene ID (NCBI) | 5091 |
| RRID | AB_2881975 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11498 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Pyruvate Carboxylase antibody 66615-1-Ig | Download protocol |
| WB protocol for Pyruvate Carboxylase antibody 66615-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Front Oncol piR-hsa-211106 Inhibits the Progression of Lung Adenocarcinoma Through Pyruvate Carboxylase and Enhances Chemotherapy Sensitivity. | ||
Elife Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells
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