Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66653-1-Ig targets IRAK1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0728 Product name: Recombinant human IRAK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 420-633 aa of BC014963 Sequence: GQRAVKTHGARTKYLVYERLEKLQAVVAGVPGHLEAASCIPPSPQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS Predict reactive species |
| Full Name | interleukin-1 receptor-associated kinase 1 |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC014963 |
| Gene Symbol | IRAK1 |
| Gene ID (NCBI) | 3654 |
| RRID | AB_2882010 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P51617 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IRAK1 antibody 66653-1-Ig | Download protocol |
| WB protocol for IRAK1 antibody 66653-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-IRAK1 antibody (catalog no. 66653-1-Ig) has 5 citations. These appear in Cell Mol Immunol, Cell Death Dis, Cancer Lett, J Cell Biol, and Biol Reprod. The research fields span innate immune signaling (IRAK1/TRAF6 activation), glioma radioresistance via autophagy, ribosome biogenesis and oncogenic translation, TLR/IL-1R pathway regulation, and reproductive biology (trophoblast function in miscarriage). Applications cited include immunofluorescence and immunohistochemistry in human samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation. | ||
Cell Death Dis Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells | ||
Cancer Lett Fibrillarin-dependent 2'-O-methylation modulates RPS28 ribosome incorporation and oncogenic translation. | ||
J Cell Biol BLK positively regulates TLR/IL-1R signaling by catalyzing TOLLIP phosphorylation | ||
Biol Reprod miR-146b-5p downregulates IRAK1 and ADAM19 to suppress trophoblast proliferation, invasion and migration in miscarriage |
Reviews
What customers say
The single review for this IRAK1 monoclonal antibody reports a 5-star rating for Western Blot. The user tested it at a 1:10,000 dilution on UKE-1 cells and found it works well in combination with HRP-linked secondary antibodies. Overall, the feedback is positive, highlighting reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Matthew (Verified Customer) (09-07-2026) | The IRAK1 monoclonal antibody works well in combination with HRP-linked secondary antibodies for Western blotting
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