Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, Sp2/0 cells, human peripheral blood platelets, HEK-293 cells, K-562, LNCaP cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66668-1-Ig targets PLCB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15845 Product name: Recombinant human PLCB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 885-1234 aa of BC142681 Sequence: AQAGQETCQDTQSQQLGSQPSSNPTPSPLDASPRRPPGPTTSPASTSLSSPGQRDDLIASILSEVAPTPLDELRGHKALVKLRSRQERDLRELRKKHQRKAVTLTRRLLDGLAQAQAEGRCRLRPGALGGAADVEDTKEGEDEAKRYQEFQNRQVQSLLELREAQVDAEAQRRLEHLRQALQRLREVVLDANTTQFKRLKEMNEREKKELQKILDRKRHNSISEAKMRDKHKKEAELTEINRRHITESVNSIRRLEEAQKQRHDRLVAGQQQVLQQLAEEEPKLLAQLAQECQEQRARLPQEIRRSLLGEMPEGLGDGPLVACASNGHAPGSSGHLSGADSESQEENTQL Predict reactive species |
| Full Name | phospholipase C, beta 3 (phosphatidylinositol-specific) |
| Calculated Molecular Weight | 1234 aa, 139 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC142681 |
| Gene Symbol | PLCB3 |
| Gene ID (NCBI) | 5331 |
| RRID | AB_2882022 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q01970 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PLCB3 antibody 66668-1-Ig | Download protocol |
| WB protocol for PLCB3 antibody 66668-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLCB3 Monoclonal antibody (Cat# 66668-1-Ig) has 7 total citations. These appear in journals including International Journal of Molecular Medicine, Ecotoxicology and Environmental Safety, International Journal of Molecular Sciences, Journal of Cellular and Molecular Medicine, Journal of Food Science, Toxicology and Applied Pharmacology, and Journal of Food Biochemistry. The associated research fields span liver disease (NAFLD), ovarian injury, lung cancer, vascular smooth muscle function, platelet activation and aggregation, and macrophage damage, with all studies employing Western blot (WB) applications across mouse, human, and rat species.
| Species | Application | Title |
|---|---|---|
Int J Mol Med Peroxisome proliferator‑activated receptor α regulates acesulfame‑K‑induced NAFLD via hepatic PLCβ: Foe and friend. | ||
Ecotoxicol Environ Saf Protective role of RGS2 in PM2.5-induced ovarian injury in rats: Modulation of Gq/11 signaling to maintain granulosa cell [Ca2 + ]i stability | ||
Int J Mol Sci Characterization of DNA Methylation Episignatures for Radon-Induced Lung Cancer | ||
J Cell Mol Med Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring. | ||
J Food Sci Tripeptide Hyp-Asp-Gly from collagen peptides inhibited platelet activation via regulation of PI3K/Akt-MAPK/ERK1/2 signaling pathway | ||
Toxicol Appl Pharmacol RGS2 attenuates alveolar macrophage damage by inhibiting the Gq/11-Ca2+ pathway during cowshed PM2.5 exposure, and aberrant RGS2 expression is associated with TLR2/4 activation |
