Tested Applications
| Positive WB detected in | A431 cells |
| Positive IHC detected in | mouse brain tissue, human thyroid cancer tissue, mouse heart tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:650-1:2600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66703-1-Ig targets THRA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27189 Product name: Recombinant human THRA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 44-282 aa of BC002728 Sequence: PSYLDKDEQCVVCGDKATGYHYRCITCEGCKGFFRRTIQKNLHPTYSCKYDSCCVIDKITRNQCQLCRFKKCIAVGMAMDLVLDDSKRVAKRKLIEQNRERRRKEEMIRSLQQRPEPTPEEWDLIHIATEAHRSTNAQGSHWKQRRKFLPDDIGQSPIVSMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPESDTLTLSGEMAV Predict reactive species |
| Full Name | thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian) |
| Calculated Molecular Weight | 490 aa, 54 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC002728 |
| Gene Symbol | THRA |
| Gene ID (NCBI) | 7067 |
| RRID | AB_3085032 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P10827 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for THRA antibody 66703-1-Ig | Download protocol |
| IHC protocol for THRA antibody 66703-1-Ig | Download protocol |
| WB protocol for THRA antibody 66703-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The THRA Monoclonal antibody (Cat# 66703-1-Ig) has 5 citations published in Advanced Science, Cell Proliferation, Molecular and Cellular Endocrinology, Theriogenology, and Journal of Muscle Research and Cell Motility. Research fields span cholangiocarcinoma photodynamic therapy, age-dependent skeletal muscle atrophy, cardiac insulin resistance, pig Sertoli cell proliferation, and skeletal muscle myoblast differentiation during aging. All citations used the antibody for Western blot applications across human, mouse, rat, and pig species.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) A NRF2 Regulated and the Immunosuppressive Microenvironment Reversed Nanoplatform for Cholangiocarcinoma Photodynamic-Gas Therapy | ||
Cell Prolif Ageing-Dependent Thyroid Hormone Receptor α Reduction Activates IP3R1-Meditated Ca(2+) Transfer in MAM and Exacerbates Skeletal Muscle Atrophy in Mice.
| ||
Mol Cell Endocrinol Exogenous Thyroxine Increases Cardiac GLUT4 Translocation in Insulin Resistant OLETF Rats | ||
Theriogenology Thyroxine regulates pig Sertoli cell line proliferation and maturation through the IKK/NFκB and p38 MAPK signaling pathways | ||
J Muscle Res Cell Motil RNA-seq and ChIP-seq unveils thyroid hormone receptor α deficiency affects skeletal muscle myoblast proliferation and differentiation via Col6a1 during aging |
