Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IHC detected in | mouse stomach tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66748-1-Ig targets KIDINS220 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17624 Product name: Recombinant human KIDINS220 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1374-1672 aa of BC130610 Sequence: LDPITEEDEKSDQSGSKLLPGKKSSERSSLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDDKDRKAEGKVERVPKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLEKANLIELEDDSHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTPSTVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRSSYTRLSKDPPELHAAASSESTGFGEERESIL Predict reactive species |
| Full Name | kinase D-interacting substrate, 220kDa |
| Calculated Molecular Weight | 1672 aa, 187 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC130610 |
| Gene Symbol | KIDINS220 |
| Gene ID (NCBI) | 57498 |
| RRID | AB_2882095 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9ULH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIDINS220 antibody 66748-1-Ig | Download protocol |
| WB protocol for KIDINS220 antibody 66748-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIDINS220 Monoclonal antibody (3G2D8), catalog number 66748-1-Ig, has 1 citation. It was published in Nature Communications (IF 18.1) in 2025, used for Western blot in human samples. The research focuses on phosphate exporter XPR1 activation and inositol pyrophosphate signaling, falling within the fields of cell signaling and phosphate metabolism.
| Species | Application | Title |
|---|---|---|
Nat Commun Synergistic activation of the human phosphate exporter XPR1 by KIDINS220 and inositol pyrophosphate |
