Tested Applications
| Positive WB detected in | Jurkat cells, PC-12 cells, HEK-293 cells, SH-SY5Y cells, Y79 cells, U-251 cells, Neuro-2a cells |
| Positive IHC detected in | mouse small intestine tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66816-1-Ig targets CHAT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19602 Product name: Recombinant human CHAT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 281-630 aa of BC130617 Sequence: SDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP Predict reactive species |
| Full Name | choline acetyltransferase |
| Calculated Molecular Weight | 748 aa, 83 kDa |
| Observed Molecular Weight | 67-71 kDa |
| GenBank Accession Number | BC130617 |
| Gene Symbol | CHAT |
| Gene ID (NCBI) | 1103 |
| RRID | AB_2882159 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P28329 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CHAT antibody 66816-1-Ig | Download protocol |
| WB protocol for CHAT antibody 66816-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHAT Monoclonal antibody (Cat# 66816-1-Ig) has 2 published citations. One appears in Neuron (2025), investigating metabolic reprogramming via histone lactylation in microglia and macrophages and its role in recruiting CD8+ T lymphocytes to aggravate spinal cord injury, using immunofluorescence in mouse. The other is in Cardiology Research and Practice (2026), examining how electroacupuncture protects against post-myocardial infarction heart failure through autonomic regulation and α7nAChR activation. The associated research fields span neuroimmunology, spinal cord injury, and cardiovascular medicine.
| Species | Application | Title |
|---|---|---|
Neuron Metabolic reprogramming through histone lactylation in microglia and macrophages recruits CD8+ T lymphocytes and aggravates spinal cord injury | ||
Cardiol Res Pract Electroacupuncture Protects Against Post-MI Heart Failure Through Autonomic Regulation and α7nAChR Activation. |
