Tested Applications
| Positive WB detected in | Jurkat cells, MCF-7 cells, HT-29 cells, K-562 cells, HEK-293 cells, Daudi cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66893-1-Ig targets TERF2 in WB, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28513 Product name: Recombinant human TERF2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-251 aa of BC024890 Sequence: MAGGGGSSDGSGRAAGRRASRSSGRARRGRHEPGLGGPAERGAGEARLEEAVNRWVLKFYFHEALRAFRGSRYGDFRQIRDIMQALLVRPLGKEHTVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTEAVVESSRKLVKEAAVIICIKNKEFEKASKILKKHMSKDPTTQKLRNDLLNIIREKNLAHPVIQNFSYETFQQKMLRFLESHLDDAEPYLLTVRLGPSPITMVCP Predict reactive species |
| Full Name | telomeric repeat binding factor 2 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 60-65.32-35 kDa |
| GenBank Accession Number | BC024890 |
| Gene Symbol | TERF2 |
| Gene ID (NCBI) | 7014 |
| RRID | AB_2882222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15554 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TERF2 antibody 66893-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TERF2 Monoclonal antibody (5B1E1), catalog number 66893-1-Ig, has 7 citations. These appear in Nature Communications, Experimental & Molecular Medicine, Nucleic Acids Research, Science Advances, Aging Cell, Frontiers in Pharmacology, and Acta Biochimica et Biophysica Sinica. Research fields span telomere biology, cellular senescence, cancer, epigenetic regulation (m6A), myocardial injury in sepsis, and alcoholic fatty liver disease. Applications cited include WB and ChIP in human and mouse models.
| Species | Application | Title |
|---|---|---|
Nat Commun Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence | ||
Nucleic Acids Res Novel telomere-targeting dual-pharmacophore dinucleotide prodrugs for anticancer therapy | ||
Sci Adv Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A). | ||
Aging Cell PRKN-Mediated Ubiquitin-Proteasome Degradation of METTL3 Promotes Cellular Senescence. | ||
Front Pharmacol Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease. |
Reviews
What customers say
The single review for this antibody is a 5-star rating. The user reports it works well for both Western Blot and Immunofluorescence on human cardiac cells. Recommended conditions include a 1:5000 dilution in 5% BSA with overnight primary incubation for Western Blot, paired with a 1:300 secondary antibody. For ICC/IHC, a 1:500 dilution in 5% Donkey Serum with overnight incubation, a 1:400 secondary (2 hours at room temperature), and 1:10000 DAPI for nuclear staining were used. Overall, the antibody performed reliably across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Refik (Verified Customer) (08-26-2025) | Antibody works well when prepared at 1:5000 dilution in 5% BSA and overnight primary incubation for Western Blot with 1:300 secondary antibody and 1:500 dilution using 5% Donkey Serum for ICC/IHC for overnight incubation with 1:400 secondary (2 hours at rtp) and 1:10000 DAPI for nuclei.
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