HEC1/HEC/NDC80 Monoclonal antibody

HEC1/HEC/NDC80 Monoclonal Antibody for WB, ELISA

Cat No. 66960-1-Ig

Clone No.2B7A8

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NDC80, 2B7A8, HEC, HEC1, HsHec1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis using 66960-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 66960-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HSC-T6 using 66960-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

HeLa cells, NCCIT cells, HSC-T6 cells, LNCaP cells, Jurkat cells, THP-1 cells

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

66960-1-Ig targets HEC1/HEC/NDC80 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8065 Product name: Recombinant human NDC80 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 290-642 aa of BC035617 Sequence: PNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE Predict reactive species
Full Name	NDC80 homolog, kinetochore complex component (S. cerevisiae)
Calculated Molecular Weight	74 kDa
Observed Molecular Weight	71-74 kDa
GenBank Accession Number	BC035617
Gene Symbol	NDC80
Gene ID (NCBI)	10403
RRID	AB_2882283
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O14777
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for HEC1/HEC/NDC80 antibody 66960-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Sci Adv Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib. Authors - Paulina Podszywałow-Bartnicka View Article
human	WB	bioRxiv Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib. Authors - Paulina Podszywałow-Bartnicka View Article