Tested Applications
| Positive WB detected in | HeLa cells, HSC-T6 cells, NIH/3T3 cells, mouse liver tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, A549 cells, HepG2 cells, PC-3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66989-1-Ig targets hIST1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28364 Product name: Recombinant human hIST1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-360 aa of BC000430 Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESMTLMLIRISLLHRLLVLDPSQKPLQSFLPDLQITMTTLSYQSCHLCQTHYQLHLLVPAPQHLKTLTLMIFPGGLKS Predict reactive species |
| Full Name | KIAA0174 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC000430 |
| Gene Symbol | hIST1 |
| Gene ID (NCBI) | 9798 |
| RRID | AB_2882306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P53990 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for hIST1 antibody 66989-1-Ig | Download protocol |
| IHC protocol for hIST1 antibody 66989-1-Ig | Download protocol |
| WB protocol for hIST1 antibody 66989-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The hIST1 Monoclonal antibody (Cat# 66989-1-Ig) has 7 total citations. These appear in Molecular Cell, EMBO Journal, Journal of Cell Biology, Advanced Biology, Molecular Biology of the Cell, bioRxiv, and Current Biology. The research fields span lysosomal damage sensing and lysophagy, ESCRT complex function, cytokinetic abscission, and general cell biology, with applications including WB, IF, IHC, and CoIP.
| Species | Application | Title |
|---|---|---|
EMBO J ATXN3 regulates lysosome regeneration after damage by targeting K48-K63-branched ubiquitin chains | ||
J Cell Biol Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters | ||
Adv Biol (Weinh) What Is the Role of Giant Endosomal Sorting Complexes Required for Transport (ESCRT) Structures in T Cell Activation? | ||
Mol Biol Cell Loss of CHMP2A implicates an ordered assembly of ESCRT-III proteins during cytokinetic abscission. | ||
bioRxiv VPS4 and CHMP7 release centromeres from the nuclear envelope for post-mitotic positioning in daughter nuclei. |
Reviews
What customers say
The single available review rates this antibody 5 out of 5 stars. The reviewer used it on BMEL cells for Western Blot and Immunofluorescence at a 1/500 dilution and described it as a "very good antibody for murine cells." Overall, the feedback is positive, highlighting reliable performance on mouse cell lines across both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
nathalie (Verified Customer) (04-24-2026) | very good antibody for murine cells
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