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TGM1 Monoclonal antibody, PBS Only (Capture) (67004-1-PBS)

TGM1 Monoclonal antibody, PBS Only (Capture)

Name: TGM1 Monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 67004-1-PBS-100UG

TGM1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 67004-1-PBS

Clone No. 3D9C11

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Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

EC:2.3.2.13, Epidermal TGase, ICR2, KTG, LI

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBIHCCytometric bead array sample testCytometric bead array

WB analysis of human saliva using 67004-1-Ig (same clone as 67004-1-PBS)
human saliva were subjected to SDS PAGE followed by western blot with 67004-1-Ig (TGM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67004-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 67004-1-Ig (same clone as 67004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67004-1-Ig (TGM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67004-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 67004-1-Ig (same clone as 67004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67004-1-Ig (TGM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67004-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP51899-1
Cytometric bead array sample test of MP51899-1, TGM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67004-1-PBS. Detection antibody: 67004-2-PBS.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP51899-2
Cytometric bead array sample test of MP51899-2, TGM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67004-1-PBS. Detection antibody: 67004-3-PBS.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP51899-1
Cytometric bead array standard curve of MP51899-1, TGM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67004-1-PBS. Detection antibody: 67004-2-PBS. Standard:Ag3905. Range: 0.781-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP51899-2
Cytometric bead array standard curve of MP51899-2, TGM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67004-1-PBS. Detection antibody: 67004-3-PBS. Standard:Ag3905. Range: 0.781-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67004-1-PBS targets TGM1 as part of a matched antibody pair:

MP51899-1: 67004-1-PBS capture and 67004-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag3905 Product name: Recombinant human TGM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 467-817 aa of BC034699 Sequence: SGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDDGSFKIVYVEEKAIGTLIVTKAISSNMREDITYLYKHPEGSDAERKAVETAAAHGSKPNVYANRGSAEDVAMQVEAQDAVMGQDLMVSVMLINHSSSRRTVKLHLYLSVTFYTGVSGTIFKETKKEVELAPGASDRVTMPVAYKEYRPHLVDQGAMLLNVSGHVKESGQVLAKQHTFRLRTPDLSLTLLGAAVVGQECEVQIVFKNPLPVTLTNVVFRLEGSGLQRPKILNVGDIGGNETVTLRQSFVPVRPGPRQLIASLDSPQLSQVHGVIQVDVAPAPGDGGFFSDAGGDSHLGETIPMASRGGA Predict reactive species
Full Name	transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
Calculated Molecular Weight	817 aa, 90 kDa
Observed Molecular Weight	90 kDa
GenBank Accession Number	BC034699
Gene Symbol	TGM1
Gene ID (NCBI)	7051
RRID	AB_2882321
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P22735
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Transglutaminase 1 is a epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelope in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.