Tested Applications
| Positive WB detected in | HUVEC cells, A549 cells, fetal human brain tissue, HeLa cells, NCI-H1299 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67023-1-Ig targets Frizzled 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4971 Product name: Recombinant human FZD9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 23-247 aa of BC026333 Sequence: LEIGRFDPERGRGAAPCQAVEIPMCRGIGYNLTRMPNLLGHTSQGEAAAELAEFAPLVQYGCHSHLRFFLCSLYAPMCTDQVSTPIPACRPMCEQARLRCAPIMEQFNFGWPDSLDCARLPTRNDPHALCMEAPENATAGPAEPHKGLGMLPVAPRPARPPGDLGPGAGGSGTCENREKFQYVEKSRSCAPRCGPGVEVFWSRRDKDFALVWMAVWSALCFFSTA Predict reactive species |
| Full Name | frizzled homolog 9 (Drosophila) |
| Calculated Molecular Weight | 591 aa, 64 kDa |
| Observed Molecular Weight | 64-70 kDa |
| GenBank Accession Number | BC026333 |
| Gene Symbol | Frizzled 9 |
| Gene ID (NCBI) | 8326 |
| RRID | AB_2882338 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O00144 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Frizzled 9 antibody 67023-1-Ig | Download protocol |
| IHC protocol for Frizzled 9 antibody 67023-1-Ig | Download protocol |
| WB protocol for Frizzled 9 antibody 67023-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Frizzled 9 Monoclonal antibody (1G9G4), catalog number 67023-1-Ig, has 2 citations. One appeared in Cell Stem Cell (2026), studying human iPSC-derived heart valve-like assembloids modeling valve development and disease pathology. The other was published in Nature Communications (2022), investigating cross-linking of the endolysosomal system to reveal flotillin structures and cargo, using the antibody for Western blot in human samples. The citations span cardiac developmental biology and cell biology of endolysosomal trafficking.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Human iPSC-derived heart valve-like assembloids model valve development and disease pathology. | ||
Nat Commun Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo |
Reviews
What customers say
The single review for this product is a 5-star rating with no written comments. The reviewer used the antibody for immunofluorescence on fibroblasts at a 1:500 dilution and included an image of the results, suggesting satisfactory performance in that application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Susanne (Verified Customer) (04-17-2023) |
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