Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, rat brain tissue, mouse brain tissue, pig brain tissue, T-47D cells, MCF-7 cells |
| Positive IHC detected in | mouse heart tissue, human rectal cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67033-1-Ig targets CYP1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13380 Product name: Recombinant human CYP1B1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-259 aa of BC012049 Sequence: MGTSLSPNDPWPLNPLSIQQTTLLLLLSVLATVHVGQRLLRQRRRQLRSAPPGPFAWPLIGNAAAVGQAAHLSFARLARRYGDVFQIRLGSCPIVVLNGERAIHQALVQQGSAFADRPAFASFRVVSGGRSMAFGHYSEHWKVQRRAAHSMMRNFFTRQPRSRQVLEGHVLSEARELVALLVRGSADGAFLDPRPLTVVAVANVMSAVCFGCRYSHDDPEFRELLSHNEEFGRTVGAGSLVDVMPWLQYFPNPVRTVFR Predict reactive species |
| Full Name | cytochrome P450, family 1, subfamily B, polypeptide 1 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC012049 |
| Gene Symbol | CYP1B1 |
| Gene ID (NCBI) | 1545 |
| RRID | AB_2882348 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16678 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP1B1 antibody 67033-1-Ig | Download protocol |
| WB protocol for CYP1B1 antibody 67033-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CYP1B1 Monoclonal antibody (1G8D4), catalog number 67033-1-Ig, has 10 citations. These appear in journals including Redox Biology, Journal of Translational Medicine, Proceedings of the National Academy of Sciences (PNAS), Journal of Agricultural and Food Chemistry, Journal of Enzyme Inhibition and Medicinal Chemistry, Food and Chemical Toxicology, Placenta, Food Science & Nutrition, and Cell. Research fields span aryl hydrocarbon receptor (AhR) signaling, redox homeostasis, mitochondrial integrity, gut microbiota–tryptophan metabolism, autoimmune disease, cancer, and stroke. Applications cited include WB and IHC in human and mouse.
| Species | Application | Title |
|---|---|---|
Redox Biol The CYP1A-HSF1 axis alleviates mitochondrial oxidative stress to limit HBO-induced lung endothelial barrier damage. | ||
J Transl Med Machine learning-based identification of kbhb-affected tumor cell subsets as prognostic and therapeutic targets in breast cancer. | ||
Proc Natl Acad Sci U S A Cytochrome P450 1B1 directs pathogenic Th17 cell generation and autoimmune disease by fine-tuning redox homeostasis and mitochondrial integrity. | ||
Proc Natl Acad Sci U S A CRISPR screens and quantitative proteomics reveal remodeling of the aryl hydrocarbon receptor-driven proteome through PARP7 activity | ||
J Agric Food Chem Lycium Barbarum Polysaccharide Attenuates Staphylococcus aureus-Induced Mastitis via the Gut Microbiota-Derived Indole-3-Propionic Acid/AhR-IL-22-STAT3 Axis. | ||
J Enzyme Inhib Med Chem Discovery of 2-phenylethyl chromones as potent and selective CYP1B1 inhibitors. |
