Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, A549 cells, NCI-H1299 cells, Caco-2 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HepG2 cells, K-562 cells, PC-12 cells, Neuro-2a cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67109-1-Ig targets CNBP in WB, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7114 Product name: Recombinant human CNBP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-170 aa of BC000288 Sequence: MSSNECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTIEATA Predict reactive species |
| Full Name | CCHC-type zinc finger, nucleic acid binding protein |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 19-25 kDa |
| GenBank Accession Number | BC000288 |
| Gene Symbol | CNBP |
| Gene ID (NCBI) | 7555 |
| RRID | AB_2882413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CNBP antibody 67109-1-Ig | Download protocol |
| WB protocol for CNBP antibody 67109-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CNBP antibody (Cat# 67109-1-Ig) has 12 citations across journals including Nature Microbiology, Cell Death & Disease, Cell Reports, Acta Pharmacologica Sinica, Journal of Medicinal Chemistry, Journal of Molecular and Cellular Cardiology, PLoS One, Virology Journal, Poultry Science, and bioRxiv. Research fields span virology (SARS-CoV-2, HBV), oncology (gastric, colorectal, melanoma, osteosarcoma), cardiology (myocardial infarction, cardiac remodeling), RNA biology (mRNA trafficking, RNA–protein interactions), and poultry science (intramuscular fat deposition).
| Species | Application | Title |
|---|---|---|
Nat Microbiol The SARS-CoV-2 RNA-protein interactome in infected human cells.
| ||
Cell Death Dis A new candidate tumor suppressor tRF-Ser inhibits gastric cancer progression by regulating the CNBP/HSPA8 axis. | ||
Acta Pharmacol Sin Non-telomeric function deficiency of TERT enhances pressure overload-induced mouse cardiac remodeling by activation of CNBP-mediated THBS3/ITGB1 pathway. | ||
Cell Rep A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions
| ||
J Med Chem Discovery of Gut-Restricted PRMT5 Inhibitors to Intercept Colorectal Cancer in Patients with Genetic Loss of Tumor Suppressor Adenomatous Polyposis Coli. | ||
J Mol Cell Cardiol Cellular nucleic acid binding protein facilitates cardiac repair after myocardial infarction by activating β-catenin signaling |
Reviews
What customers say
The single review for this product reports a 5-star rating from a Western Blot application on 293T cells at a 1:5000 dilution. The user noted that the band was clear, indicating satisfactory performance and specificity for their experiment. Overall, the feedback is positive, reflecting reliable results in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Katherine (Verified Customer) (09-25-2023) | The band was clear
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