Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67310-1-Ig targets Entactin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.
| Tested Reactivity | Human, Pig, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4739 Product name: Recombinant human NID1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 28-400 aa of BC045606 Sequence: CLSRQELFPFGPGQGDLELEDGDDFVSPALELRGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESIENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVH Predict reactive species |
| Full Name | nidogen 1 |
| Calculated Molecular Weight | 1247 aa, 136 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC045606 |
| Gene Symbol | Entactin/NID |
| Gene ID (NCBI) | 4811 |
| RRID | AB_2882570 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P14543 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Entactin antibody 67310-1-Ig | Download protocol |
| IHC protocol for Entactin antibody 67310-1-Ig | Download protocol |
| WB protocol for Entactin antibody 67310-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Entactin Monoclonal antibody (2A11G1), catalog number 67310-1-Ig, has 1 citation. It appears in the International Journal of Molecular Sciences (2025), in a study examining Nidogen-1's role in KMT2A-rearranged pediatric acute myeloid leukemia. The associated research field is oncology, specifically pediatric leukemia and extracellular matrix biology.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
Customers report strong performance for this NID1 antibody in immunohistochemistry and immunofluorescence. One user tested it alongside several other NID1 antibodies and found it performed best, with specific staining consistent with published literature. Another user, also comparing multiple NID1 antibodies, described it as the cleanest and best-looking result they received. Both used a 1:1000 dilution on placental tissue. Overall, reviewers highlight its specificity, clean staining, and superior performance relative to comparable NID1 antibodies.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Avelina (Verified Customer) (04-02-2025) | We titrated 5 different NID1 antibodies and this one performed the best. Current staining appears specific and relevant to what we find in literature.
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Jillian (Verified Customer) (03-27-2025) | We tested multiple NID1 antibodies and this was the cleanest and best looking one that we recieved
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