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Alpha SNAP Monoclonal antibody (67323-1-Ig)

Alpha SNAP Monoclonal antibody

Alpha SNAP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67323-1-Ig

Clone No. 1E2A2

Publications (1)

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Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

Alpha SNAP, NAPA, SNAP alpha, SNAPA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

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Filter: AllWBIHCIF-P

WB analysis using 67323-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 67323-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 67323-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 67323-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 67323-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 67323-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Alpha SNAP in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	pig brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected in	mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67323-1-Ig targets Alpha SNAP in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity	Human, Pig, Mouse, Rat
Cited Reactivity	mouse
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag13180 Product name: Recombinant human NAPA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-295 aa of BC007432 Sequence: MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR Predict reactive species
Full Name	N-ethylmaleimide-sensitive factor attachment protein, alpha
Calculated Molecular Weight	33 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC007432
Gene Symbol	Alpha SNAP
Gene ID (NCBI)	8775
RRID	AB_2882583
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P54920
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for Alpha SNAP antibody 67323-1-Ig	Download protocol
IHC protocol for Alpha SNAP antibody 67323-1-Ig	Download protocol
WB protocol for Alpha SNAP antibody 67323-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

I'll look up the citation information for this antibody.The Alpha SNAP Monoclonal antibody (catalog 67323-1-Ig, clone 1E2A2) has 1 total citation. The citation appears in the journal Scientific Reports (2025). The study, titled "αSnap plays a pivotal role in the maintenance of Drosophila ISCs survival and tissue homeostasis," demonstrates the antibody's use in mouse samples by Western blot (WB), associating the citation with the research field of intestinal stem cell (ISC) survival and tissue homeostasis.