Tested Applications
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HepG2 cells, LNcaP cells, Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67374-2-Ig targets BRD4 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27545 Product name: Recombinant human BRD4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 311-633 aa of BC035266 Sequence: PEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRV Predict reactive species |
| Full Name | bromodomain containing 4 |
| Calculated Molecular Weight | 1362 aa, 152 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC035266 |
| Gene Symbol | BRD4 |
| Gene ID (NCBI) | 23476 |
| RRID | AB_2918488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O60885 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BRD4 antibody 67374-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BRD4 Monoclonal antibody (Cat# 67374-2-Ig) has 16 citations published in journals including Cell, Nature Methods, Nature Communications, Cell Death & Disease, Cell Reports, BMC Biology, European Journal of Medicinal Chemistry, Journal of Proteome Research, and others. Research fields span epigenetics, transcriptional regulation, cancer biology (breast, prostate, colorectal, lung, gastric, bladder, glioblastoma, thyroid, and head & neck cancers), ferroptosis, drug discovery, immune evasion, and inflammatory responses. Applications include WB, ChIP, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Cell De novo assembly of nuclear stress bodies rearranges and enhances NFIL3 to restrain acute inflammatory responses | ||
Nat Methods High-throughput profiling of chemical-induced gene expression across 93,644 perturbations | ||
Nat Commun Proteome-wide ligandability maps of drugs with diverse cysteine-reactive chemotypes | ||
Nat Commun MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition | ||
Cell Death Dis (+)-JQ-1 alleviates cardiac injury in myocardial infarction by inhibiting ferroptosis through the NAMPT/SIRT1 pathway. | ||
J Transl Med Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation |
