Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, Neuro-2a cells, MOLT-4 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67410-1-Ig targets MEK1/2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29871 Product name: Recombinant human MAP2K2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-400 aa of BC018645 Sequence: MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKELEAIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQPGTPTRTAV Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase 2 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44-47 kDa |
| GenBank Accession Number | BC018645 |
| Gene Symbol | MEK2 |
| Gene ID (NCBI) | 5605 |
| RRID | AB_2882651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P36507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 67410-1-Ig can recognize both the MEK2 and MEK1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MEK1/2 antibody 67410-1-Ig | Download protocol |
| IHC protocol for MEK1/2 antibody 67410-1-Ig | Download protocol |
| WB protocol for MEK1/2 antibody 67410-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MEK1/2 Monoclonal antibody (catalog number 67410-1-Ig) has 14 citations. These appear in journals including Signal Transduction and Targeted Therapy, Nature Microbiology, Science Translational Medicine, Advanced Science, Cell Reports Medicine, Cell Death & Disease, PNAS, Frontiers in Medicine, Frontiers in Immunology, Ecotoxicology and Environmental Safety, Journal of Clinical and Translational Hepatology, mBio, PNAS Nexus, and Molecular and Cellular Biochemistry. Research fields span cancer biology (colorectal, liver, and pancreatic cancers), signaling pathways (ERK/MAPK), immunology, toxicology, and infectious disease.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther IGF-1R inhibition induces MEK phosphorylation to promote survival in colon carcinomas. | ||
Sci Transl Med Phase separation-based HTS identifies cobimetinib as a YAP-TEAD inhibitor that suppresses hyperactivated YAP-induced cancer progression | ||
Adv Sci (Weinh) Icaritin Represses Autophagy to Promote Colorectal Cancer Cell Apoptosis and Sensitized Low-Temperature Photothermal Therapy via Targeting HSP90-TXNDC9 Interactions | ||
Cell Rep Med Nilotinib induces immunogenic cuproptosis to potentiate cancer immunotherapy. | ||
Cell Death Dis OTUD6B-mediated K48 deubiquitination of FXR1 forms a positive feedback loop activating MEK2/ERK signaling in colorectal cancer liver metastasis. |
