Tested Applications
| Positive WB detected in | HuH-7 cells, rat liver tissue |
| Positive IHC detected in | mouse eye tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HuH-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67438-1-Ig targets PROX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9681 Product name: Recombinant human PROX1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 402-737 aa of BC024201 Sequence: FHTANQRLQCFGDVIIPNPLDTFGNVQMASSTDQTEALPLVVRKNSSDQSASGPAAGGHHQPLHQSPLSATTGFTTSTFRHPFPLPLMAYPFQSPLGAPSGSFSGKDRASPESLDLTRDTTSLRTKMSSHHLSHHPCSPAHPPSTAEGLSLSLIKSECGDLQDMSEISPYSGSAMQEGLSPNHLKKAKLMFFYTRYPSSNMLKTYFSDVKFNRCITSQLIKWFSNFREFYYIQMEKYARQAINDGVTSTEELSITRDCELYRALNMHYNKANDFEVPERFLEVAQITLREFFNAIIAGKDVDPSWKKAIYKVICKLDSEVPEIFKSPNCLQELLHE Predict reactive species |
| Full Name | prospero homeobox 1 |
| Calculated Molecular Weight | 737 aa, 83 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC024201 |
| Gene Symbol | PROX1 |
| Gene ID (NCBI) | 5629 |
| RRID | AB_2882674 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92786 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PROX1 antibody 67438-1-Ig | Download protocol |
| IHC protocol for PROX1 antibody 67438-1-Ig | Download protocol |
| WB protocol for PROX1 antibody 67438-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PROX1 Monoclonal antibody (1D4B1), catalog number 67438-1-Ig, has 4 cited publications. These appear in Journal of Translational Medicine, Developmental Cell, Inflammation Research, and Development. The research fields span oncology (lymphatic metastasis in head and neck squamous cell carcinoma), neuroscience (neocortical and hippocampal development, neurogenesis), and renal/lymphatic biology (aldosterone-induced renal lymphangiogenesis). Applications cited include WB and IHC in human samples.
| Species | Application | Title |
|---|---|---|
J Transl Med Metabolic expression profiling analysis reveals pyruvate-mediated EPHB2 upregulation promotes lymphatic metastasis in head and neck squamous cell carcinomas | ||
Dev Cell Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells | ||
Inflamm Res Aldosterone induces renal lymphangiogenesis through macrophage-lymphatic endothelial cell transformation and Inhibition by esaxerenone | ||
Development KLF7 orchestrates hippocampal development through neurogenesis and Draxin-mediated neuronal migration |
Reviews
What customers say
The single review for this antibody reports a 5-star rating for Immunofluorescence. The user tested it at a 1:500 dilution on both human and murine tissue samples, noting that it "worked really well" in both species. Overall, the feedback is positive, highlighting reliable performance across species in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Julia (Verified Customer) (05-07-2026) | worked really well in human and murin samples
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