Tested Applications
| Positive WB detected in | A-253 cells, SW 1990 cells, MDA-MB-231 cells, SCaBER cells, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67556-1-Ig targets RIG-1/DDX58 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig, duck |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18591 Product name: Recombinant human DDX58 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 575-925 aa of BC132786 Sequence: RAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Calculated Molecular Weight | 925 aa, 106 kDa |
| Observed Molecular Weight | 101/106 kDa |
| GenBank Accession Number | BC132786 |
| Gene Symbol | RIG-1/DDX58 |
| Gene ID (NCBI) | 23586 |
| RRID | AB_2882770 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95786 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58,which has two isoform with the MW of 106 and 101 kDa.
Publications
What published studies show
The RIG-1/DDX58 Monoclonal antibody (Cat# 67556-1-Ig) has 17 citations spanning journals including Immunity, Nature Communications, Cell Reports, Autophagy, Redox Biology, Advanced Science, Journal of Virology, PLoS Pathogens, BMC Cancer, Virulence, Journal of Translational Medicine, International Journal of Biological Macromolecules, Emerging Microbes & Infections, Investigative Ophthalmology & Visual Science, Poultry Science, and Veterinary Microbiology. Research fields cover innate/antiviral immunity, interferon signaling, autophagy, ferroptosis, viral replication, and cancer biology.
| Species | Application | Title |
|---|---|---|
Immunity Flavin adenine dinucleotide is an endogenous suppressor for cytosolic DNA and RNA sensors to modulate innate immunity. | ||
Redox Biol Nlrp6 protects from corticosterone-induced NSPC ferroptosis by modulating RIG-1/MAVS-mediated mitophagy | ||
Adv Sci (Weinh) API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs | ||
J Transl Med Truncated APC impairs innate immune response by targeting MAVS on mitochondria in colorectal cancer. |
