Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, human placenta tissue, rat retina tissue, mouse brain tissue, JAR cells, Pig brain tissue, Rat brain tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67565-1-Ig targets PDE6D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29563 Product name: Recombinant human PDE6D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of NM_002601 Sequence: MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV Predict reactive species |
| Full Name | phosphodiesterase 6D, cGMP-specific, rod, delta |
| Calculated Molecular Weight | 17 KDa |
| Observed Molecular Weight | 15-17 kDa |
| GenBank Accession Number | NM_002601 |
| Gene Symbol | PDE6D |
| Gene ID (NCBI) | 5147 |
| RRID | AB_2882779 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43924 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDE6D antibody 67565-1-Ig | Download protocol |
| WB protocol for PDE6D antibody 67565-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDE6D Monoclonal antibody (Cat# 67565-1-Ig) has 1 citation to date. It appears in Cancer Letters (2025), a study investigating how metformin inhibits castration-resistant prostate cancer progression by regulating PDE6D-induced purine metabolic alternation and cGMP/PKG pathway activation. The antibody was applied in WB and IHC on human and mouse samples. The associated research field is oncology, specifically prostate cancer metabolism and signaling.
| Species | Application | Title |
|---|---|---|
Cancer Lett Metformin inhibits the progression of castration resistant prostate cancer by regulating PDE6D induced purine metabolic alternation and cGMP / PKG pathway activation
|
Reviews
What customers say
Both reviews (Western Blot, 3/5 stars) confirm the antibody is functional but produces noticeable background and nonspecific bands. One user found it "very noisy" yet still effective when validating PDE6 delta knockout cells at 1:2000 dilution. The other reported similar background issues but achieved the best results using 5% milk in TBS-T for blocking, primary, and secondary steps rather than 3% BSA in TBS-T, at a 1:1000 dilution. Overall, users suggest optimizing blocking conditions to reduce nonspecific signal.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Charlotte (Verified Customer) (07-24-2023) | We tested the antibody against PDE6 delta KO cells. We can see the antibody is very noisy but it is working.
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Criseyda (Verified Customer) (09-19-2022) | The antibody had background bands/nonspecific bands present but worked best in 5% Milk TBS-T (Blocking, Primary and Secondary) and not 3% BSA TBS-T (Blocking, Primary and Secondary).
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