Tested Applications
| Positive WB detected in | K-562 cells, HEK-293 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
67592-1-Ig targets TERF1 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12540 Product name: Recombinant human TERF1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 118-419 aa of BC029378 Sequence: CQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED Predict reactive species |
| Full Name | telomeric repeat binding factor (NIMA-interacting) 1 |
| Calculated Molecular Weight | 439 aa, 50 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | BC029378 |
| Gene Symbol | TERF1 |
| Gene ID (NCBI) | 7013 |
| RRID | AB_2918494 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P54274 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Telomeres are nucleoprotein complexes located at chromosome ends that consist of tandem arrays of TTAGGG repeats bound to specific proteins. Telomeres protect chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. TERF1 (telomeric repeat-binding factor 1) binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. It's one component of the shelterin complex, which involved in the regulation of telomere length and protection.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TERF1 antibody 67592-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Refik (Verified Customer) (08-22-2025) | Antibody works really well at a 1:250 - 500 dilution for ICC imaging of 2D and 3D cardiac cells with secondary Alexa Flour antibody at 1:400 - 500 and DAPI for both human and mouse specific cells. Works really well at 1:1000 concentration in western blot for overnight incubation followed by 1:300 secondary antibody incubation for 1 hour for human and mouse tissue.
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