Tested Applications
| Positive WB detected in | LNCaP cells, HEK-293 cells, NIH/3T3 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67701-1-Ig targets HNRNPF in WB, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7128 Product name: Recombinant human HNRNPF protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-415 aa of BC001432 Sequence: MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD Predict reactive species |
| Full Name | heterogeneous nuclear ribonucleoprotein F |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC001432 |
| Gene Symbol | HNRNPF |
| Gene ID (NCBI) | 3185 |
| RRID | AB_2882893 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P52597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HNRNPF antibody 67701-1-Ig | Download protocol |
| WB protocol for HNRNPF antibody 67701-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HNRNPF Monoclonal antibody (Cat# 67701-1-Ig) has 14 citations across journals including Signal Transduction and Targeted Therapy, Journal of Hepatology, Oncogene, Cell Reports, Genes Discovery, Drug Resistance Updates, Clinical and Experimental Medicine, RNA Biology, Frontiers in Physiology, Acta Pharmacologica Sinica, Asian Journal of Andrology, Acta Biochimica et Biophysica Sinica, and bioRxiv. Research fields span prostate cancer ferroptosis, cholangiocarcinoma, bladder/breast/lung cancer, HCC, spermatogenesis, TNFα/NFκB signaling, RNA biology, and ER stress.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Enhancer transcription profiling reveals an enhancer RNA-driven ferroptosis and new therapeutic opportunities in prostate cancer | ||
J Hepatol Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD | ||
Drug Resist Updat Lactate-driven H3K18 lactylation promotes cisplatin resistance in bladder cancer via HNRNPF-Parkin mediated mitophagy. | ||
Genes Dis Nuclear and cytoplasmic USP30-AS1 coordinately regulate breast cancer progression through HnRNPF/p21 and EZH2/c-Myc/p21 axes.
| ||
Oncogene Super-enhancer-associated long noncoding RNA lnc-SPI1U mediates SPI1 feedback regulation by interacting with HNRNPH1 and HNRNPF. | ||
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The customer used it for Western Blot on Lymphocyte T cells at a 1:2000 dilution and found it worked well, noting that the recommended dilution of 1:5000 was perfect for detection. Overall, the feedback indicates reliable performance and effective target detection in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Celine (Verified Customer) (02-10-2026) | The recommended dilution of 1/5000 was perfect for the detection.
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