Tested Applications
| Positive WB detected in | HepG2 cells, NCCIT cells, PC-3 cells, DU 145 cells |
| Positive IHC detected in | human placenta tissue, human colon cancer tissue, human liver cancer tissue, human lung cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67766-1-Ig targets gp130/IL6ST in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28808 Product name: Recombinant human IL-6ST protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 124-369 aa of NM_002184 Sequence: MLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVT Predict reactive species |
| Full Name | interleukin 6 signal transducer (gp130, oncostatin M receptor) |
| Calculated Molecular Weight | 104 kDa |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | NM_002184 |
| Gene Symbol | IL6ST |
| Gene ID (NCBI) | 3572 |
| RRID | AB_2918533 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P40189 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for gp130/IL6ST antibody 67766-1-Ig | Download protocol |
| WB protocol for gp130/IL6ST antibody 67766-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The gp130/IL6ST Monoclonal antibody (1H7C5), catalog number 67766-1-Ig, has 12 citations. These appear in journals including Nature Biotechnology, Advanced Science, International Journal of Biological Sciences, Journal of Translational Medicine, Developmental Cell, Cell Proliferation, International Immunopharmacology, Frontiers in Cell and Developmental Biology, Journal of Inflammation Research, Frontiers in Physiology, Cancer Medicine, and PLoS ONE. Research fields span IL-6/STAT3 signaling, cancer (colorectal, glioblastoma, pancreatic, breast, liposarcoma), autophagy, RNA-protein interactions, sepsis, cartilage development, gastric inflammation, and endometriosis.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) BLOC1S1 Attenuates B. Melitensis 16M LPS-Triggered Autophagy by Spatial Confinement of TDP-43. | ||
Int J Biol Sci GPX1 expression promotes stemness and aggressiveness in myxoid liposarcomas | ||
J Transl Med Positive feedback loop of IGF2BP3/IL6ST/STAT3 facilitates malignant progression in colorectal cancer.
| ||
Dev Cell STAT3-controlled CHI3L1/SPP1 positive feedback loop demonstrates the spatial heterogeneity and immune characteristics of glioblastoma | ||
Cell Prolif Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling. |
Reviews
What customers say
The single review for this product reports a 5-star rating for Western Blot. The user used a 1:1000 dilution on human T-cells and noted "nice results" for the Western Blot application. Overall, the feedback is positive, indicating the antibody performed well in its tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jimmy (Verified Customer) (05-28-2025) | Nice results for western blot application
|
