Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, SH-SY5Y cells, pig brain tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67821-1-Ig targets PPARGC1B in WB, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
| Tested Reactivity | Human, mouse, pig, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28861 Product name: Recombinant human PPARGC1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC132971 Sequence: MAGNDCGALLDEELSSFFLNYLADTQGGGSGEEQLYADFPELDLSQLDASDFDSATCFGELQWCPENSETEPNQYSPDDSELFQIDSENEALLAELTKTLDDIPEDDVGLAAFPALDGGDALSCTSASPAPSSAPPSPAPEKPSAPAPEVDELSLLQKLLLATSYPTSSSDTQKEGTAWRQAGLRSKSQRPCVKADSTQDKKAPMMQSQSRSCTELHKHLTSAQCCLQDRGLQPPCLQSPRLPAKEDKEPGEDCPSPQPAPASPRDSLALGRADPGAPVSQEDMQAMVQLIRYMHTYCLPQRKLPPQTPEPLPKACSNPSQQVRSRPWSRHHSKASWAEFSILRELLAQD Predict reactive species |
| Full Name | peroxisome proliferator-activated receptor gamma, coactivator 1 beta |
| Calculated Molecular Weight | 1023 aa, 113 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC132971 |
| Gene Symbol | PPARGC1B |
| Gene ID (NCBI) | 133522 |
| RRID | AB_2918584 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q86YN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPARGC1B antibody 67821-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPARGC1B Monoclonal antibody (2D2E8), catalog number 67821-1-Ig, has a total of 2 citations. These citations appear in Chemical & Biological Interactions and Scientific Reports. The associated research fields include cadmium-induced kidney injury and sirtuin 1-P53 signaling pathway activation, as well as dietary oleic acid effects on skeletal muscle fiber type composition. Both studies utilized the antibody for Western blot applications in rat and mouse models, respectively.
| Species | Application | Title |
|---|---|---|
Chem Biol Interact N-acetylcysteine delayed cadmium-induced chronic kidney injury by activating the sirtuin 1-P53 signaling pathway | ||
Sci Rep Dietary oleic acid intake increases the proportion of type 1 and 2X muscle fibers in mice |
