Tested Applications
| Positive WB detected in | HEK-293 cells, LNCaP cells, Jurkat cells, K-562 cells, Daudi cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67856-1-Ig targets RCHY1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4942 Product name: Recombinant human RCHY1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-261 aa of BC047393 Sequence: MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSQQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ Predict reactive species |
| Full Name | ring finger and CHY zinc finger domain containing 1 |
| Calculated Molecular Weight | 261 aa, 30 kDa |
| Observed Molecular Weight | 45 KDa, 30 kDa |
| GenBank Accession Number | BC047393 |
| Gene Symbol | RCHY1 |
| Gene ID (NCBI) | 25898 |
| RRID | AB_2918614 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96PM5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RCHY1 antibody 67856-1-Ig | Download protocol |
| IHC protocol for RCHY1 antibody 67856-1-Ig | Download protocol |
| WB protocol for RCHY1 antibody 67856-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RCHY1 Monoclonal antibody (2C11C11), catalog number 67856-1-Ig, has a total of 1 citation. It appears in the journal iScience (2023), in a study investigating AKT1- and TRIM21-mediated phosphodegron control of proteasomal degradation of HuR and its role in cell survival under heat shock. The associated research fields include protein degradation, heat shock response, and cell survival signaling.
| Species | Application | Title |
|---|---|---|
iScience An AKT1-and TRIM21-mediated phosphodegron controls proteasomal degradation of HuR enabling cell survival under heat shock
|
