CDK7 Monoclonal antibody

CDK7 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67889-1-Ig

Clone No. 1E7B6

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

39 kDa protein kinase, CAK, CAK1, CDK activating kinase, CDK7, CDKN7, Cell division protein kinase 7, cyclin dependent kinase 7, MO15, p39 Mo15, p39MO15, STK1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIHCIF/ICC

WB analysis using 67889-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 67889-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 67889-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 67889-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 67889-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A431 using 67889-1-Ig
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, U2OS cells, MCF-7 cells, Hela cells, Jurkat cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67889-1-Ig targets CDK7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag25737 Product name: Recombinant human CDK7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 183-345 aa of BC005298 Sequence: LLFGARMYGVGVDMWAVGCILAELLLRVPFLPGDSDLDQLTRIFETLGTPTEEQWPDMCSLPDYVTFKSFPGIPLHHIFSAAGDDLLDLIQGLFLFNPCARITATQALKMKYFSNRPGPTPGCQLPRPNCPVETLKEQSNPALAIKRKRTEALEQGGLPKKLI Predict reactive species
Full Name	cyclin-dependent kinase 7
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC005298
Gene Symbol	CDK7
Gene ID (NCBI)	1022
RRID	AB_2918645
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P50613
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CDK7 antibody 67889-1-Ig	Download protocol
IHC protocol for CDK7 antibody 67889-1-Ig	Download protocol
WB protocol for CDK7 antibody 67889-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The CDK7 Monoclonal antibody (1E7B6), catalog number 67889-1-Ig, has 1 citation. It appears in Frontiers in Cell and Developmental Biology (2025), in a study investigating keloid pathogenesis and the biomarker potential of CDK7 and DDB2. The cited applications were Western blot and immunofluorescence in human samples.