MPG Monoclonal antibody

MPG Monoclonal Antibody for WB, ELISA

Cat No. 67920-1-Ig

Clone No. 2C12F3

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, ELISA

Synonyms

3 alkyladenine DNA glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, MPG, N methylpurine DNA glycosylase, PIG11, PIG16

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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WB analysis using 67920-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67920-1-Ig (MPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 67920-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67920-1-Ig (MPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for MPG in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67920-1-Ig targets MPG in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity	Human, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28647 Product name: Recombinant human MPG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-293 aa of BC014991 Sequence: MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA Predict reactive species
Full Name	N-methylpurine-DNA glycosylase
Calculated Molecular Weight	293 aa, 32 kDa
Observed Molecular Weight	35-36 kDa
GenBank Accession Number	BC014991
Gene Symbol	MPG
Gene ID (NCBI)	4350
RRID	AB_2918672
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P29372
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MPG antibody 67920-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The MPG monoclonal antibody (clone 2C12F3), catalog number 67920-1-Ig, has 1 total citation. It was cited in Science Advances (IF: 13.9) in a 2023 study investigating how mammalian STN1 deficiency promotes colon cancer development by inhibiting DNA repair. The research field encompasses cancer biology and DNA repair mechanisms, with the antibody used for Western blot in human samples.