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FARSB Monoclonal antibody

FARSB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67924-1-Ig
Clone No. 1H10C2
Publications (1)
Review Product
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Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
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  • Western Blot (WB) analysis of various lysates using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HepG2 cells using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis of HepG2 using 67924-1-Ig

    WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HepG2 cells.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HeLa cells using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis of HeLa using 67924-1-Ig

    WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HeLa cells.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HEK-293 cells using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis of HEK-293 using 67924-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of various lysates using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of various lysates using FARSB Monoclonal antibody (67924-1-Ig)

    WB analysis using 67924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse brain tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse brain using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse brain tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse brain using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using FARSB Monoclonal antibody (67924-1-Ig)

    IHC staining of mouse cerebellum using 67924-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FARSB Monoclonal antibody (67924-1-Ig)

    IF Staining of HeLa using 67924-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using FARSB Monoclonal antibody (67924-1-Ig)

    IF Staining of U-251 using 67924-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67924-1-Ig targets FARSB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9695

Product name: Recombinant human FARSB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 240-589 aa of BC017783

Sequence: DHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINIGPFL

Predict reactive species
Full Name phenylalanyl-tRNA synthetase, beta subunit
Calculated Molecular Weight 589 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC017783
Gene Symbol FARSB
Gene ID (NCBI) 10056
RRIDAB_2918676
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NSD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for FARSB antibody 67924-1-IgDownload protocol
IHC protocol for FARSB antibody 67924-1-IgDownload protocol
WB protocol for FARSB antibody 67924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The FARSB Monoclonal antibody (1H10C2), catalog number 67924-1-Ig, has 1 total citation. The citation appears in Nan Fang Yi Ke Da Xue Xue Bao (2023), where the antibody was used for IHC to study FARSB's role in stratifying prognosis and the cold tumor microenvironment across different cancer types via integrated single-cell and bulk RNA sequencing analysis. The research field is oncology/tumor microenvironment characterization.

Automatically generated from the 1 publication citations by Able AI.
SpeciesApplicationTitle
human,mouseIHC

Nan Fang Yi Ke Da Xue Xue Bao

[FARSB stratifies prognosis and cold tumor microenvironment across different cancer types: an integrated single cell and bulk RNA sequencing analysis]

Authors - Ziran Zhang
View Article
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