Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells, rabbit brain tissue, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68042-1-Ig targets PAIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rabbit samples.
| Tested Reactivity | human, mouse, rabbit |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16773 Product name: Recombinant human PAIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 102-400 aa of BC005295 Sequence: EQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ Predict reactive species |
| Full Name | poly(A) binding protein interacting protein 1 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 40-45kDa, 62kDa |
| GenBank Accession Number | BC005295 |
| Gene Symbol | PAIP1 |
| Gene ID (NCBI) | 10605 |
| RRID | AB_2918784 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H074 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAIP1 antibody 68042-1-Ig | Download protocol |
| WB protocol for PAIP1 antibody 68042-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PAIP1 Monoclonal antibody (2H9D4), catalog number 68042-1-Ig, has 1 citation. It appears in Science Advances (2025), in a study investigating how fibronectin in olfactory mucus modulates olfactory receptor sensitivity to odorants. The antibody was used for IHC on human samples, placing this citation in the research fields of olfactory biology and neuroscience.
| Species | Application | Title |
|---|---|---|
Sci Adv Fibronectin in the olfactory mucus increases sensitivity of olfactory receptor response to odorants |
