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NSFL1C Monoclonal antibody, PBS Only (Capture) (68182-1-PBS)

Filter: AllWBIHCIF/ICCCytometric bead array sample testCytometric bead array

WB analysis using 68182-1-Ig (same clone as 68182-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.
WB analysis using 68182-1-Ig (same clone as 68182-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 68182-1-Ig (same clone as 68182-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 68182-1-Ig (same clone as 68182-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 68182-1-Ig (same clone as 68182-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSFL1C antibody (68182-1-Ig, Clone: 1F12E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51764-1
Cytometric bead array sample test of MP51764-1, NSFL1C Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68182-1-PBS. Detection antibody: 68182-2-PBS.
Cytometric bead array standard curve of MP51764-1
Cytometric bead array standard curve of MP51764-1, NSFL1C Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68182-1-PBS. Detection antibody: 68182-2-PBS. Standard:Ag8040. Range: 0.781-100 ng/mL.

NSFL1C Monoclonal antibody, PBS Only (Capture)

Name: NSFL1C Monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 68182-1-PBS

NSFL1C Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 68182-1-PBS

Clone No.1F12E12

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

1F12E12, NSFL1 cofactor p47, p97 cofactor p47, UBX domain-containing protein 2C, UBXN2C

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

68182-1-PBS targets NSFL1C as part of a matched antibody pair:

MP51764-1: 68182-1-PBS capture and 68182-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8040 Product name: Recombinant human NSFL1C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-370 aa of BC002801 Sequence: MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT Predict reactive species
Full Name	NSFL1 (p97) cofactor (p47)
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	41 kDa
GenBank Accession Number	BC002801
Gene Symbol	NSFL1C
Gene ID (NCBI)	55968
RRID	AB_2935273
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9UNZ2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.