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  • Western Blot (WB) analysis of various lysates using SGF29 Monoclonal antibody (68339-1-Ig)

    WB analysis using 68339-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68339-1-Ig (CCDC101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

SGF29 Monoclonal antibody

SGF29 Monoclonal Antibody for WB, ELISA
Cat No. 68339-1-Ig
Clone No.1G1B4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Synonyms

CCDC101, FLJ32446, SGF29

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68339-1-Ig targets SGF29 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SGF29 fusion protein Ag33219 Predict reactive species
Full Name coiled-coil domain containing 101
Calculated Molecular Weight 293 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC011981
Gene Symbol SGF29
Gene ID (NCBI) 112869
RRIDAB_3085063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96ES7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.

Protocols

Product Specific Protocols
WB protocol for SGF29 antibody 68339-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols