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What's the best dilution for MYH6 in ?
Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68461-1-Ig targets MYH6 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 6, cardiac muscle, alpha |
| Calculated Molecular Weight | 224 kDa |
| Observed Molecular Weight | 200-220 kDa |
| GenBank Accession Number | NM_002471 |
| Gene Symbol | MYH6 |
| Gene ID (NCBI) | 4624 |
| RRID | AB_3085173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P13533 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH6 antibody 68461-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYH6 Monoclonal antibody (1A5E5), catalog number 68461-1-Ig, has a total of 2 citations. These appear in Cell Death Dis and Am J Physiol Heart Circ Physiol. The cited research fields include cardiac fibroblast maturation and paracrine signaling involving long non-coding RNA (Charme), as well as maternal cardiac remodeling associated with hypercholesterolemia during pregnancy. Both studies utilized the antibody for Western blot applications in mouse and rat models.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Effect of the cardiac long non-coding RNA Charme depletion on the maturation and paracrine signaling of resident cardiac fibroblasts. | ||
Am J Physiol Heart Circ Physiol Excessive hypercholesterolemia in pregnancy alters maternal cardiac remodeling during pregnancy and postpartum. |
