Tested Applications
| Positive WB detected in | A431 cells, NIH/3T3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68497-1-Ig targets BMPR2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5868 Product name: Recombinant human BMPR2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 172-504 aa of BC052985 Sequence: YRMLTGDRKQGLHSMNMMEAAASEPSLDLDNLKLLELIGRGRYGAVYKGSLDERPVAVKVFSFANRQNFINEKNIYRVPLMEHDNIARFIVGDERVTADGRMEYLLVMEYYPNGSLCKYLSLHTSDWVSSCRLAHSVTRGLAYLHTELPRGDHYKPAISHRDLNSRNVLVKNDGTCVISDFGLSMRLTGNRLVRPGEEDNAAISEVGTIRYMAPEVLEGAVNLRDCESALKQVDMYALGLIYWEIFMRCTDLFPGESVPEYQMAFQTEVGNHPTFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETIEDCWDQDAEARLTAQCAEERMAEL Predict reactive species |
| Full Name | bone morphogenetic protein receptor, type II (serine/threonine kinase) |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 120-140 kDa |
| GenBank Accession Number | BC052985 |
| Gene Symbol | BMPR2 |
| Gene ID (NCBI) | 659 |
| RRID | AB_3085208 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13873 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. The dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form, and 150 kD dimer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BMPR2 antibody 68497-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BMPR2 Monoclonal antibody (2C1D7), catalog number 68497-1-Ig, has 1 citation. It appears in the journal Lab Invest (Impact Factor 4.1), published in November 2025. The cited study investigates how Leptin Receptor-expressing fibroblasts activated by BMP signaling promote bone resorption in developmental odontogenic cysts, placing the research in the fields of bone biology, BMP signaling, and odontogenic pathology. The application used was immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
Lab Invest Leptin Receptor-Expressing (LepR(+)) Fibroblasts Activated by BMP Signaling Promote Bone Resorption in Developmental Odontogenic Cysts. |
