HLA-DRA Monoclonal antibody

HLA-DRA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68543-1-Ig

Clone No. 3D6E8

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3D6E8, HLA DRA, HLA DRA1, HLA DRB, HLA-DR

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 68543-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68543-1-Ig (HLA-DRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse lung using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat lung using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat skin using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 68543-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Raji using 68543-1-Ig
Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRA antibody (68543-1-Ig, Clone: 3D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Raji cells, Daudi cells, Ramos cells, pig lung tissue
Positive IHC detected in	human tonsillitis tissue, rat skin tissue, human stomach cancer tissue, mouse lung tissue, mouse skin tissue, human rectal cancer tissue, rat lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68543-1-Ig targets HLA-DRA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag11224 Product name: Recombinant human HLA-DRA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 24-218 aa of BC032350 Sequence: WAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENV Predict reactive species
Full Name	major histocompatibility complex, class II, DR alpha
Calculated Molecular Weight	254 aa, 29 kDa
Observed Molecular Weight	32-34 kDa
GenBank Accession Number	BC032350
Gene Symbol	HLA-DRA
Gene ID (NCBI)	3122
ENSEMBL Gene ID	ENSG00000204287
RRID	AB_3085250
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P01903
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for HLA-DRA antibody 68543-1-Ig	Download protocol
IHC protocol for HLA-DRA antibody 68543-1-Ig	Download protocol
WB protocol for HLA-DRA antibody 68543-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The HLA-DRA Monoclonal antibody (3D6E8), catalog number 68543-1-Ig, has 1 total citation. The cited publication appeared in PLoS One (2025) and is associated with glioma immunology research, specifically examining CAF subtypes and apCAF-M2 macrophage interactions driving immune resistance. The antibody was used for IF (immunofluorescence) in this study.

Species	Application	Title
	IF	PLoS One A multi-omics atlas of CAF subtypes reveals apCAF-M2 macrophage interactions driving immune resistance in glioma Authors - Yubo Ren View Article