Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, NK-92 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68547-1-Ig targets SKIV2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33679 Product name: Recombinant human SKIV2L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 861-1246 aa of BC015758 Sequence: QVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPEGPCDHTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLAQAHPAGPPTLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQYLKLRERMQIQKEMERLRFLLSDQSLLLLPEYHQRVEVLRTLGYVDEVGTVKLAGRVACAMSSHELLLTELMFDNALSTLRPEEIAALLSGLVCQSPGDAGDQLPNTLKQGIERVRAVAKRIGEVQVACGLNQTVEEFVGELNFGLVEVVYEWARGMPFSELAGLSGTPEGLVVRCIQRLAEMCRSLRGAARLVGEPVLGAKMETAATLLRRDIVFAASLYTQ Predict reactive species |
| Full Name | superkiller viralicidic activity 2-like (S. cerevisiae) |
| Calculated Molecular Weight | 1246 aa, 138 kDa |
| Observed Molecular Weight | 138-145 kDa |
| GenBank Accession Number | BC015758 |
| Gene Symbol | SKIV2L |
| Gene ID (NCBI) | 6499 |
| RRID | AB_3085252 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15477 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SKIV2L antibody 68547-1-Ig | Download protocol |
| IHC protocol for SKIV2L antibody 68547-1-Ig | Download protocol |
| WB protocol for SKIV2L antibody 68547-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
Both reviews (5/5 stars) report successful Western Blot performance. One user detected specific Skiv2l bands in RKO cells at a 1:2000 dilution, while another confirmed a band at the expected molecular weight in U2OS cells at 1:3000. Overall, customers highlight the antibody's specificity and reliable detection across different human cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aleksandr (Verified Customer) (07-29-2025) | Specific detection of human Skiv2l in RKO cells
 |
Tsimafei (Verified Customer) (11-19-2024) | Antibody detect a band of the correct molecular weight in U2OS cells
 |
