Tested Applications
| Positive WB detected in | Saos-2 cells, MDA-MB-231 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human stomach cancer, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68888-1-Ig targets Histone H2A.X in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | H2A histone family, member X |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC013416 |
| Gene Symbol | Histone H2A.X |
| Gene ID (NCBI) | 3014 |
| RRID | AB_3670446 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P16104 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phases. It is involved in nucleosomal organization of chromatin together with other histone proteins and is especially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Histone H2A.X antibody 68888-1-Ig | Download protocol |
| IF protocol for Histone H2A.X antibody 68888-1-Ig | Download protocol |
| IHC protocol for Histone H2A.X antibody 68888-1-Ig | Download protocol |
| WB protocol for Histone H2A.X antibody 68888-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Histone H2A.X Monoclonal antibody (3E11B2), catalog number 68888-1-Ig, has 17 citations. These appear in journals including Int J Biol Sci, J Immunother Cancer, Phytomedicine, Adv Healthc Mater, Mater Today Bio, Acta Pharmacol Sin, Alzheimers Res Ther, J Med Chem, Cell Signal, Mol Oncol, iScience, BMC Cancer, Reprod Toxicol, Histochem Cell Biol, Res Sq, and Neurooncol Adv. Research fields span DNA damage response, cancer/chemo-immunotherapy, ferroptosis, radiation biology, Alzheimer's/tauopathies, and cell signaling.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Targeting Ash1L-STING Axis Restores NK Cell Function and Ameliorates Immune-Mediated Bone Marrow Failure Diseases. | ||
J Immunother Cancer Targeting ATF4 overcomes dual resistance to chemo-immunotherapy in gastric cancer by disrupting the DNA damage response. | ||
Phytomedicine Pharmacological targeting of NR4A1 restrains lipid metabolism-ferroptosis axis in UVR-induced skin aging. | ||
Adv Healthc Mater Shewanella-Augmented Cerium Oxide Nanoclusters With Superior Antioxidant Activity for Enhanced Radioprotection of Intestine. | ||
Mater Today Bio A multifunctional parathyroid hormone-related supramolecular peptide-loaded dual network hydrogel for radiation-induced wound repair | ||
Acta Pharmacol Sin Alkalization of the intracellular pH reprograms TNF-α signaling from inflammatory NF-κB activation to tumoricidal RIP kinase-dependent necroptosis in cancer cells. |
Reviews
What customers say
Both reviewers rated this antibody 5 out of 5 stars for Western Blot. One user employed a 1:5,000 dilution on HeLa cells and described it as a good antibody. The other used a 1:1,000 dilution on MDA-MB-231 cells and reported an excellent experience. Overall, customers found the antibody reliable and effective for WB applications across different cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arnaud (Verified Customer) (08-04-2026) | Good antibody
|
Md Asad (Verified Customer) (06-11-2026) | Excellent experience.
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